Description
Aviva Systems Biology Tri-iodothyronine ELISA Kit (Goat) (OKCA00274) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Goat Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tri-iodothyronine and incubated. The Tri-iodothyronine found in the sample or standards competes with the biotinylated Tri-iodothyronine for limited binding sites on the immobilized anti-Goat Tri-iodothyronine antibody. Excess unbound biotinylated Tri-iodothyronine and sample or standard Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tri-iodothyronine captured in the well and inversely proportional to the amount of Tri-iodothyronine which was contained in the sample or standard